Biological Information | |
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Background Information: | PathHunter Pathway Reporter cell lines are engineered to express an Enzyme Donor (ED) tagged reporter protein controlled by a pathway-inducible transcriptional response element. Pathway activation results in induced expressions of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer, lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal. |
Target Class: | Signaling Pathway |
Family: | Tumor Necrosis Factor Receptor Superfamily |
Accession Number: | NC_000004.12 |
Target Name: | NF-kB |
Target Aliases: | KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta |
Target Species: | Human |
Cell Background: | HEK293 |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Signaling Reporters |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Signaling |
Clinical Relevance | |
Therapeutic Area: | Inflammation/Allergy |
Additional Information | |
Brand: | PathHunter® |
PathHunter® NF-kB Pathway Reporter Cell Line (HEK293)
The PathHunter Signaling Pathway Reporter Assays are stable clonal cell lines that monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology.
User Manuals & Protocols
70-406 PH Signaling Pathway Reporter Assay User Manual_REV0
View DocumentDatasheets
93-1164_NFKB(HEK)_Reporter_DS_02142024
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