PathHunter® MC2R β-Arrestin Cell Line (U2OS)

The PathHunter® MC2R β-Arrestin Cell Line (U2OS) measures MC4R (GPCR) activity via recruitment of β-Arrestin 2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1189C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® MC2R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC2R GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged MC2R and EA-tagged β-Arrestin 2. Activation of MC4R stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Melanocortin
Coupling:
Gs
Accession Number:
NM_005912.2
Target Name:
MC2R
Target Aliases:
ACTH receptor, ACTH-R, Adrenocorticotropin receptor, Melanocortin receptor 2 (MC2-R), Adrenocorticotropic hormone receptor
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
?-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-247-PathHunter-Beta-Arrestin-Assay-for-GPCR-Cell-Lines_REV5-1 User Manual

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Datasheets

93-1189C3 Datasheet

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