PathHunter® HTR2C Activated GPCR Internalization U2OS Cell Line

The PathHunter® HTR2C Activated GPCR Internalization U2OS Cell Line measures HTR2C (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0669C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® HTR2C Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based internalization of HTR2C via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged HTR2C. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting HTR2C-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	5-Hydroxytryptamine Receptors (Serotonin)
Sub Family:	Class A
Coupling:	Gq
Accession Number:	NM_000868.2
Target Name:	HTR2C (5-HT2C receptor)
Target Aliases:	5-hydroxytryptamine receptor 2C, 5-HT1C, 5-HT2C, 5-HTR2C, 5HTR2C, HTR1C
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®