PathHunter® HEK 293 ADRA1D β-Arrestin Cell line

The PathHunter® HEK 293 ADRA1D β-Arrestin Cell line measures ADRA1D (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0983C1 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® ADRA1D cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1D GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged ADRA1D and EA-tagged β-Arrestin 2. Activation of ADRA1D stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Adrenoceptor
Sub Family:	Class A
Coupling:	Gq
Accession Number:	NM_000678
Target Name:	ADRA1D (α1D-adrenoceptor)
Target Aliases:	Adrenergic receptor alpha 1D, DAR, ADRA1, ADRA1A, ADRA1R, ALPHA1
Target Species:	Human
Cell Background:	HEK293

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	β-Arrestin Recruitment
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®