Biological Information | |
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Background Information: | The PathHunter® F2RL3 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. serine proteases, protease-activated receptor 4) based internalization of F2RL3 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged F2RL3. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting F2RL3-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Proteinase-Activated Receptors |
Coupling: | Gi/Go & Gq |
Accession Number: | AY431102 |
Target Name: | F2RL3 (PAR4) |
Target Aliases: | Coagulation factor II (thrombin) receptor-like 3 (PAR4), PAR-4, PAR4, coagulation factor II (thrombin) receptor-like 3, coagulation factor II receptor-like 3, protease-activated receptor-4, proteinase-activated receptor 4, proteinase-activated receptor-4, thrombin receptor-like 3 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
β-Arrestin isoform: | β-Arrestin-2 |
Assay Measures: | Internalization |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® F2RL3 Activated GPCR Internalization U2OS Cell Line
The PathHunter® F2RL3 Activated GPCR Internalization U2OS Cell Line measures F2RL3 (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-259 PathHunter Activated GPCR Internalization Assay REV6 User Manual
View DocumentDatasheets
93-0753C3 Datasheet
View Document