Biological Information | |
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Background Information: | The PathHunter® CXCR1 (IL8RA) Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. CXCL8/IL-8) based internalization of CXCR1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR1 (IL8RA). Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR1 (IL8RA)-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Sub Family: | Class A |
Coupling: | Gi/Go |
Accession Number: | NM_000634 |
Target Name: | CXCR1 (IL8RA) |
Target Aliases: | Chemokine (C-X-C motif) receptor 1, C-C, C-C-CKR-1, CD128, CD181, CDw128a, CKR-1, CMKAR1, IL8R1, IL8RA, IL8RBA, CXCR1, IL8RA, Chemokine |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
β-Arrestin isoform: | β-Arrestin-2 |
Assay Measures: | Internalization |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® CXCR1 (IL8RA) Activated GPCR Internalization U2OS Cell Line
The PathHunter® CXCR1 (IL8RA) Activated GPCR Internalization U2OS Cell Line measures CXCR1 (IL8RA) (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-259 PathHunter Activated GPCR Internalization Assay REV6 User Manual
View DocumentDatasheets
93-0659C3 Datasheet
View Document