PathHunter® CMKLR1 Activated GPCR Internalization U2OS Cell Line

The PathHunter® CMKLR1 Activated GPCR Internalization U2OS Cell Line measures CMKLR1 (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0720C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® CMKLR1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. chemerin) based internalization of CMKLR1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CMKLR1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CMKLR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Chemerin Receptors
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_004072.2
Target Name:	CMKLR1
Target Aliases:	Chemokine-like receptor 1, CHEMERINR, ChemR23, DEZ, ERV1, RVER1
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®