| Biological Information | |
|---|---|
| Background Information: | The PathHunter® PROKR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PROKR2 and EA-tagged β-Arrestin 2. Activation of PROKR2 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
| Target Class: | GPCR |
| Family: | Prokineticin Receptors |
| Sub Family: | Class A |
| Accession Number: | NM_144773 |
| Target Name: | PROKR2 |
| Target Aliases: | Prokineticin receptor 2, GPR73L1, GPR73b, GPRg2, HH3, KAL3, PKR2, dJ680N4.3 |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | Stable Cell Lines |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | β-Arrestin Recruitment |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® CHO-K1 PROKR2 β-Arrestin Cell Line
The PathHunter® CHO-K1 PROKR2 β-Arrestin Cell Line measures PROKR2 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual
View DocumentDatasheets
93-0282C2 Datasheet
View Document