Biological Information | |
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Background Information: | The PathHunter® PPARδ Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. fatty acids, L-165041) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PPARδ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of PPARδ, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Nuclear Protein |
Family: | NHR |
Sub Family: | Non-Steroid |
Accession Number: | NM_177435 |
Target Name: | PPARδ |
Target Aliases: | Peroxisome Proliferator Activated Receptor delta |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Protein Interaction |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® CHO-K1 PPARδ Protein Interaction Cell Line
The PathHunter® CHO-K1 PPARδ Protein Interaction Cell Line measures activated PPARδ nuclear hormone receptor interaction with SRCPs. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-263 PathHunter NHR Assays REV6 User Manual
View DocumentDatasheets
93-0492C2 Datasheet
View Document