| Biological Information | |
|---|---|
| Background Information: | The PathHunter® CHO-K1 MRGPRX2 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. cortistatin 14) based activation of MRGPRX2 GPCR via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged MRGPRX2 and EA-tagged β-Arrestin-2. Activation of MRGPRX2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility, and faster implementation from characterization to lot release. |
| Target Class: | GPCR |
| Family: | Class A Orphans |
| Sub Family: | Class C |
| Accession Number: | NM_054030.2 |
| Target Name: | MRGPRX2 |
| Target Aliases: | Mas-related GPR, member X2, MGRG3, MRGX2 |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | β-Arrestin Recruitment |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® CHO-K1 MRGPRX2 Bioassay Kit
The PathHunter® CHO-K1 MRGPRX2 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. cortistatin 14) based activation of MRGPRX2 GPCR via detection of β-arrestin recruitment.
User Manuals & Protocols
70-419 PathHunter CHO-K1 MRGPRX2 Bioassay Kit User Manual REV0
View DocumentDatasheets
93-0309Y2x Datasheet
View Document