PathHunter® CHO-K1 LGR5 (GPR49) β-Arrestin Orphan GPCR Cell Line

The PathHunter® CHO-K1 LGR5 (GPR49) β-Arrestin Orphan GPCR Cell Line measures LGR5 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0526C2A Size: 2 vials

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Biological Information
Background Information:	The PathHunter® LGR5 (GPR49) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. R-spondins) based activation of LGR5 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged LGR5 and EA-tagged β-Arrestin 2. Activation of LGR5 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Class A Orphans
Accession Number:	NM_003667
Target Name:	LGR5 (GPR49)
Target Aliases:	Leucine-rich repeat-containing G protein-coupled receptor 5, FEX, GPR49, GPR67, GRP49, HG38
Target Species:	Human
Cell Background:	CHO-K1

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	β-Arrestin Recruitment
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®