PathHunter® CHO-K1 HTR1A β-Arrestin Cell Line

The PathHunter® CHO-K1 HTR1A β-Arrestin Cell Line measures HTR1A (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0696C2 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® HTR1A cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR1A GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged HTR1A and EA-tagged β-Arrestin 2. Activation of HTR1A stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	5-Hydroxytryptamine Receptors (Serotonin)
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_000524.2
Target Name:	HTR1A (5-HT1A receptor)
Target Aliases:	5-hydroxytryptamine receptor 1A, 5-HT-1A, 5-HT1A, 5-HT1a receptor, 5-hydroxytryptamine (serotonin) receptor 1A, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled, 5-hydroxytryptamine receptor 1A, 5HT1a, ADRB2RL1, ADRBRL1, G protein coupled receptor, G-21, guanine nucleotide-binding regulatory protein-coupled receptor, serotonin receptor 1A
Target Species:	Human
Cell Background:	CHO-K1

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	β-Arrestin Recruitment
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®