Biological Information | |
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Background Information: | The PathHunter® CALCR-RAMP2(AMY2) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin, amylin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CALCR and EA-tagged β-Arrestin 2. Activation of CALCR stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Both calcitonin and amylin activate amylin receptors (AMY1, AMY2, AMY3). Amylin, a 37-amino acid peptide hormone, is primarily synthesized in pancreatic beta cells and co-secreted with insulin in response to ingested nutrients. Amylin receptors are GPCRs composed of two units: a core unit formed by the calcitonin receptor (a 7-transmembrane receptor) and one of three receptor activity-modifying proteins (RAMP1-3). Dual amylin and calcitonin receptor agonists have shown significant efficacy in promoting body weight loss, enhancing glucose tolerance, and reducing fat accumulation in liver tissue. Moreover, Amylin and GLP-1R agonists are believed to function via comparable yet distinct mechanisms, particularly in regulating appetite. Consequently, Amylin-based therapeutics exhibit promising potential in combating obesity. |
Target Class: | GPCR |
Family: | Calcitonin Receptor |
Sub Family: | Class B |
Coupling: | Gs |
Accession Number: | NM_001742.2 |
Target Name: | CALCR-RAMP2(AMY2) |
Target Aliases: | Calcitonin receptor, CRT, CT-R, CTR, CTR1, Amylin receptor, AMY2 |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
β-Arrestin isoform: | β-Arrestin-2 |
Assay Measures: | β-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® CHO-K1 CALCR-RAMP2 (AMY2) β-Arrestin Cell Line
The PathHunter® CHO-K1 CALCR-RAMP2 (AMY2) β-Arrestin Cell Line measures CALCR (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual
View DocumentDatasheets
93-0267C2 Datasheet
View Document