PathHunter® BHK-21 rPTGER4 β-Arrestin Cell Line

The PathHunter® BHK-21 rPTGER4 β-Arrestin Cell Line measures rPTGER4 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells. Rat protein.

Catalog #: 93-0908C16 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® rPTGER4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. [ligand]) based activation of rPTGER4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged rPTGER4 and EA-tagged β-Arrestin2. Activation of rPTGER4 stimulates the recruitment of β-Arrestin2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein.
Target Class:	GPCR
Family:	Prostanoid Receptors
Coupling:	Gs
Accession Number:	NM_032076
Target Name:	PTGER4
Target Aliases:	Prostaglandin E receptor 4 (subtype EP4), rat
Target Species:	Rat
Cell Background:	BHK-21

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	β-Arrestin Recruitment
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®