PathHunter® AGTRL1 Activated GPCR Internalization U2OS Cell Line

The PathHunter® AGTRL1 Activated GPCR Internalization U2OS Cell Line measures AGTRL1 (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0710C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® AGTRL1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. apelin-13) based internalization of AGTRL1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Apelin Receptor
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_005161
Target Name:	AGTRL1
Target Aliases:	APLNR, Apelin receptor, APJ, APJR, HG11, AGTRL1
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	Internalization
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®