| Biological Information | |
|---|---|
| Background Information: | ADCP is the mechanism by which antibody-opsonized target cells activate the FcγRs on the surface of macrophages to induce phagocytosis, resulting in internalization and degradation of the target cell through phagosome acidification. In the KILR Daudi ADCP Bioassay, ready-to-use KILR Daudi target cells expressing the antigens of interest are co-incubated with macrophages and an opsonizing therapeutic antibody for 24 hours. In this assay, KILR Daudi target cells endogenously expressing the desired antigen (e.g. CD19, CD20 or CD38) were engineered to stably express a housekeeping protein that is tagged with enhanced ProLabel® (ePL), a β-gal reporter fragment. These KILR Daudi target cells will be phagocytosed by macrophages during the process of ADCP. The remaining target cells in the co-culture are lysed in the presence of PathHunter® PL/PK Detection reagent kit which includes the EA fragment of β-gal enzyme, and enzyme substrate, resulting in complementation of any remaining KILR reporter protein in which produces a functional β-galactosidase enzyme, which generates a chemiluminescent signal that can be detected on any benchtop luminescence reader. |
| Target Name: | CD19, CD20, CD38 |
| Target Species: | Human |
| Cell Background: | Daudi |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Cytotoxicity |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | KILR® |
KILR® Daudi ADCP Bioassay Kit
KILR Daudi ADCP Bioassay kit provides a functional, robust, highly sensitive, and easy-to-use-cell based assay to study drug potency and neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including KILR Daudi Bioassay cryopreserved cells. This bioassay has been optimized for a 96-well format.
User Manuals & Protocols
70-442 KILR Daudi ADCP Bioassay Kit User Manual REV0
View DocumentDatasheets
97-1009Y025x Datasheet
View Document