InCELL Hunter™ ULK1 Kinase Cell Line (HEK 293)

The InCELL Hunter™ ULK1 Kinase Binding Cell Line (HEK293) is a stable, engineered cell line for use in studying  small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of ULK1 via detection of ULK1 levels. This product uses EFC technology. The cell line included overexpresses ULK1 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog #: 96-0081C1 Size: 2 vials
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Biological Information
Background Information:
The InCELL Hunter™ ULK1 Kinase Binding Cell Line (HEK293) is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of ULK1 via detection ofULK1 levels. This product uses EFC technology. The cell line included overexpresses ULK1 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Kinase
Sub Family:
Protein Ser/Thr
Accession Number:
NP_003556.2
Target Name:
ULK1
Target Aliases:
UNC51-LIKE KINASE 1, UNC-51 like autophagy activating Kinase 1
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Target Engagement
Detection Method:
Chemiluminescence
Additional Information
Brand:
InCELL Hunter™

User Manuals & Protocols

70-400_InCELL Hunter_Target_Engagement_Cell Lines_REV0 User Manual

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Datasheets

96-0081C1 Datasheet

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