Biological Information | |
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Background Information: | The InCELL Hunter™ HEK 293 PIKFYVE Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of PIKFYVE via detection of PIKFYVE levels. This product uses EFC technology. The cell line included overexpresses PIKFYVE target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Kinase |
Target Name: | PIKFYVE |
Target Aliases: | phosphoinositide kinase, FYVE-type zinc finger containing, CFD, FAB1, HEL37, PIP5K, PIP5K3, ZFYVE29 |
Cell Background: | HEK293 |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Target Engagement |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | InCELL Hunter™ |
InCELL Hunter™ HEK 293 PIKFYVE Kinase Binding Cell Line
The InCELL Hunter™ HEK 293 PIKFYVE Kinase Binding Cell Line measures target engagement via compound-induced stabilization of PIKFYVE target protein. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-400 InCELL Hunter Target Engagement Cell Lines REV0 User Manual
View DocumentDatasheets
96-0078C1 Datasheet
View Document