Biological Information | |
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Background Information: | The ADRA1A Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1A , a naturally Gq coupled GPCR. Upon activation of ADRA1A , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Adrenoceptor |
Sub Family: | Class A |
Coupling: | Gq |
Accession Number: | NM_000680 |
Target Name: | ADRA1A (α1A-adrenoceptor) |
Target Aliases: | Adrenergic receptor alpha 1A, ADRA1A, α1A, Adrenoceptor |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Calcium Flux |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | DiscoverX |
CHO-K1 ADRA1A Gq Cell Line
The CHO-K1 ADRA1A Gq Cell Line measures ADRA1A (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-296 Native Calcium GPCR Assays REV2 User Manual
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95-1023C2 Datasheet
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