Biological Information | |
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Background Information: | The ChemiScreen™ OXGR1 α-Ketoglutarate Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-ketoglutarate) based activation of OXGR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OXGR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of OXGR1 , the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Oxoglutarate Receptor |
Accession Number: | NM_080818.3 |
Target Name: | OXGR1 (GPR80, GPR99) |
Target Aliases: | OXGR1, GPR99/GPR80, Oxoglutarate |
Target Species: | Human |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Measures: | Calcium Flux |
Detection Method: | Fluorescence |
Additional Information | |
Brand: | ChemiSCREEN™ |
ChemiScreen™ OXGR1 alpha-Ketoglutarate Receptor Stable Cell Line
The ChemiScreen™ OXGR1 α-Ketoglutarate Receptor Stable Cell Line measures OXGR1 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
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HTS191C Datasheet
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