ChemiScreen™ MRGPRD Receptor Stable Cell Line

The ChemiScreen™ MRGPRD Receptor Stable Cell Line measures MRGPRD (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.

Catalog #: HTS206C Size: 2 vials

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Biological Information
Background Information:	The ChemiScreen™ MRGPRD Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. β-alanine) based activation of MRGPRD GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MRGPRD alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MRGPRD , the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Class A Orphans
Target Name:	MRGPRD
Target Aliases:	MRGPRD, MRGPRD, Liganded Orphan
Target Species:	Human

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Measures:	Calcium Flux
Detection Method:	Fluorescence

Additional Information
Brand:	ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS206C Datasheet

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