Biological Information | |
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Background Information: | The ChemiScreen™ FPRL1 N-Formylpeptide Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. N-formyl peptides) based activation of FPR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FPR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of FPR2 , the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Formylpeptide Receptors |
Accession Number: | NM_001462 |
Target Name: | FPR2 |
Target Aliases: | FPR2, FPRL1/ALX, Formylpeptide |
Target Species: | Human |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Measures: | Calcium Flux |
Detection Method: | Fluorescence |
Additional Information | |
Brand: | ChemiSCREEN™ |
ChemiScreen™ FPRL1 N-Formylpeptide Receptor Stable Cell Line
The ChemiScreen™ FPRL1 N-Formylpeptide Receptor Stable Cell Line measures FPR2 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
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HTS056C Datasheet
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