ChemiScreen™ beta1 Adrenoceptor Receptor Stable Cell Line

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The ChemiScreen™ β1 Adrenoceptor Receptor Stable Cell Line measures ADRB1 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.

Catalog #: HTS104C Size: 2 vials
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Biological Information
Background Information:
The ChemiScreen™ β1 Adrenoceptor Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRB1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRB1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of ADRB1 , the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Adrenoceptor
Accession Number:
NM_000684
Target Name:
ADRB1 (β1-adrenoceptor)
Target Aliases:
Adrenergic receptor beta 1, ADRB1R, RHR, B1AR, FNSS2, ADRB1R, BETA1AR
Target Species:
Human
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS104C Datasheet

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