ChemiScreen™ 5-HT2A Serotonin Receptor Stable Cell Line

The ChemiScreen™ 5-HT2A Serotonin Receptor Stable Cell Line measures HTR2A (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.

Catalog #: HTS082C Size: 2 vials

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Biological Information
Background Information:	The ChemiScreen™ 5-HT2A Serotonin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR2A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR2A alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HTR2A , the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	5-Hydroxytryptamine Receptors (Serotonin)
Accession Number:	NM_000621
Target Name:	HTR2A (5-HT2A receptor)
Target Aliases:	5-Hydroxytryptamine receptor 2A, serotonin 5HT-2 receptor, serotonin receptor 2A, HTR2A, 5HT2A, 5-Hydroxytryptamine (Serotonin)
Target Species:	Human

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Measures:	Calcium Flux
Detection Method:	Fluorescence

Additional Information
Brand:	ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS082C Datasheet

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