ChemiBrite™ GPBA Bile Acid Receptor Stable Cell Line

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The ChemiBRITE™ GPBA Bile Acid Receptor Stable Cell Line measures GPBAR1 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.

Catalog #: HTS238L Size: 2 vials
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Biological Information
Background Information:
The ChemiBrite GPBA Bile Acid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. lithocholic acid, other bile acids) based activation of GPBAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GPBAR1 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GPBAR1 , the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Bile Acid Receptor (GPBA)
Target Name:
GPBAR1
Target Aliases:
GPBAR1, GPR131, Bile Acid (GPBA)
Target Species:
Human
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiBrite™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS238L Datasheet

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