| Biological Information | |
|---|---|
| Background Information: | The ChemiBrite EP1 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 , a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER1 , the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. |
| Target Class: | GPCR |
| Family: | Prostanoid Receptors |
| Accession Number: | NM_000955.2 |
| Target Name: | PTGER1 |
| Target Aliases: | Prostaglandin E receptor 1 (subtype EP1), PTGER1, EP1, Prostanoid |
| Target Species: | Human |
| Usage | |
| Product Type: | Stable Cell Lines |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Calcium Flux |
| Detection Method: | Fluorescence |
| Additional Information | |
| Brand: | ChemiBrite™ |
ChemiBrite™ EP1 Prostanoid Receptor Stable Cell Line
The ChemiBRITE™ EP1 Prostanoid Receptor Stable Cell Line measures PTGER1 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
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HTS099L Datasheet
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