Biological Information | |
---|---|
Background Information: | The ChemiBrite CXCR3 Chemokine Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of CXCR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of CXCR3 , the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Accession Number: | NM_001504.1 |
Target Name: | CXCR3 (IP-10) |
Target Aliases: | Chemokine (C-X-C motif) receptor 3, CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR |
Target Species: | Human |
Cell Background: | HEK293 |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Measures: | Calcium Flux |
Detection Method: | Fluorescence |
Additional Information | |
Brand: | ChemiBrite™ |
ChemiBrite™ CXCR3 Chemokine Receptor Stable Cell Line
The ChemiBRITE™ CXCR3 Chemokine Receptor Stable Cell Line measures CXCR3 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
View DocumentDatasheets
HTS003L Datasheet
View Document