Biological Information | |
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Background Information: | The PathHunter® U2OS GHSR Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand based activation of GHSR GPCR via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged GHSR and EA-tagged β-Arrestin-2. Activation of GHSR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Ghrelin Receptor |
Accession Number: | NM_198407 |
Target Name: | GHSR |
Target Aliases: | Ghrelin Receptor, GHDP |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Bioassay Kits |
Application: | Potency; Commercial Release & Stability |
Kit Components: | |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | β-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS GHSR Bioassay Kit
User Manuals & Protocols
70-418 PathHunter U2OS GHSR Bioassay Kit User Manual REV0
View DocumentDatasheets
93-0242Y3x Datasheet
View Document