An integrative biology approach for analysis of drug action in models of human vascular inflammation.

Authors: Kunkel EJ, Dea M, Ebens A, Hytopoulos E, Melrose J, Nguyen D, Ota KS, Plavec I, Wang Y, Watson SR, Butcher EC, Berg EL.
Publisher/Year: FASEB Journal. 2004;18(11):1279–1281.
Pub Med ID/Journal ID: PMID: 15208272


Validation of compounds for activities relevant to human disease biology is a major bottleneck in drug development. Here we apply statistical analyses to protein expression data sets from primary human cell-based systems in multiple complex environments relevant to vascular inflammation to detect and distinguish compound mechanisms. We show that this approach, termed biologically multiplexed activity profiling (BioMAP profiling), is able to discriminate the action of diverse compound classes, identify off-target or secondary activities, and lead to insights into clinical activities of compounds.