[User Manual] PathHunter® NHR Assay
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Nuclear Hormone Receptor (NHR) activation is comprised of four steps: 1) ligand binding to the NHR, 2) nuclear translocation, 3) recruitment of a Steroid Receptor Coactivator Peptide (SRCP), and 4) transcriptional activation of target DNA sequence. PathHunter products represent novel cell-based applications of the established Enzyme Fragment Complementation (EFC) technology pioneered by DiscoveRx. PathHunter NHR assays were developed using EFC with two different approches, Nuclear Translocation and Protein Interaction.