Features & Benefits
- Single functional readout – applicable to different GPCRs, NHRs and kinase receptors and families
- Stable cell lines - increased reliability and reproducibility
- Full length protein – ideal for large molecule therapeutics and small molecule inhibitors
- High specificity – tagged protein eliminates background from endogenous proteins
- High quality reproducible data – superior assay windows & performance (Z' > 0.6)
- Simple readout using standard plate readers
Explore Target Biology With Multiple Technology Platforms
A homogenous cell-based assay format to detect in vivo interaction of two proteins where the ProLink fragment is attached to one protein and the EA is attached to the second protein. Active β-gal enzyme is formed only when the two proteins interact with each other. The robust assays are specific, sensitive and highthroughput adaptable and use the EFC technology.
Learn more about PathHunter Protein:protein Interaction Assays >
PathHunter cells are genetically engineered to over-express the protein under study fused to PL/PK and the EA localized to one of the following organelles: nucleus, cell membrane, or endosome. Upon compound stimulation of cells, the PL/PK- tagged protein undergoes translocation into the cell compartment in which the EA-is localized. This results in the formation of the active β-gal enzyme which can be detected using a chemiluminescent substrate.
Learn more about PathHunter Protein Translocation Assays >
PathHunter protein degradation assays involve a protein of interest tagged with a small ProLink peptide. The assays measure specific ProLink-tagged protein degradation in response to pathway stimulation.
Learn more about PathHunter Protein Degradation Assays >