Apoptosis Pathway

Cancer cells are known to over-express a number of anti-apoptotic proteins that belong to the BCL2 family, on the surface of the mitochondria. These proteins interact with pro-apoptotic proteins and regulate the intrinsic apoptotic pathway by controlling the mitochondrial membrane permeability and release of the pro-apoptotic factor; Cytochrome c which initiates activation of the Caspase cascade and apoptosis.

Eurofins DiscoverX now offers a novel, direct, cell-based assays to study these apoptotic players. No more assay development required; you can obtain pre-validated biosensor cell lines that allows you to screen for protein interaction inhibitors (small molecules or siRNA) in a HTS-friendly and simple assay format!

 

How it Works


 

Features and Benefits
 

  • Simple, HTS-friendly protocol

  • Whole cell, chemiluminescent assay

  • Avoid antibodies and lengthy FLOW based assay development


Applications
 

  • Broad applicability

  • Screen for small molecule inhibitors of protein interaction

  • Screen for siRNA

 

Assay List

Cell Lines

Target

Description

Cat. No.

Bcl2-Bax

InCELL Hunter™ U2OS Bcl2-Bax Protein Binding Cell Line

96-0010C3

Bcl2-Bim

InCELL Hunter™ U2OS Bcl2-Bim Protein Binding Cell Line

96-0008C3

Bcl2L1-Bax

InCELL Hunter™ U2OS Bcl2L1-Bax Protein Binding Cell Line

96-0009C3

Bcl2L1-Bim

InCELL Hunter™ U2OS Bcl2L1-Bim Protein Binding Cell Line

96-0037C3


Assay Ready Kits

Target

Description

Cat. No.

Bcl2L1-Bax

InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay

96-0009E3CP16L

Bcl2L1-Bax

InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay

96-0009E3CP16M

Bcl2L1-Bax

InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay

96-0009E3CP16S