Apoptosis Pathway
Cancer cells are known to over-express a number of anti-apoptotic proteins that belong to the BCL2 family, on the surface of the mitochondria. These proteins interact with pro-apoptotic proteins and regulate the intrinsic apoptotic pathway by controlling the mitochondrial membrane permeability and release of the pro-apoptotic factor; Cytochrome c which initiates activation of the Caspase cascade and apoptosis.
Eurofins DiscoverX now offers a novel, direct, cell-based assays to study these apoptotic players. No more assay development required; you can obtain pre-validated biosensor cell lines that allows you to screen for protein interaction inhibitors (small molecules or siRNA) in a HTS-friendly and simple assay format!
How it Works
Features and Benefits
-
Simple, HTS-friendly protocol
-
Whole cell, chemiluminescent assay
-
Avoid antibodies and lengthy FLOW based assay development
Applications
Assay List
Cell Lines
Target
|
Description
|
Cat. No.
|
Bcl2-Bax
|
InCELL Hunter™ U2OS Bcl2-Bax Protein Binding Cell Line
|
96-0010C3
|
Bcl2-Bim
|
InCELL Hunter™ U2OS Bcl2-Bim Protein Binding Cell Line
|
96-0008C3
|
Bcl2L1-Bax
|
InCELL Hunter™ U2OS Bcl2L1-Bax Protein Binding Cell Line
|
96-0009C3
|
Bcl2L1-Bim
|
InCELL Hunter™ U2OS Bcl2L1-Bim Protein Binding Cell Line
|
96-0037C3
|
Assay Ready Kits
Target
|
Description
|
Cat. No.
|
Bcl2L1-Bax
|
InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay
|
96-0009E3CP16L
|
Bcl2L1-Bax
|
InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay
|
96-0009E3CP16M
|