Apoptosis Pathway
Cancer cells are known to over-express a number of anti-apoptotic proteins that belong to the BCL2 family, on the surface of the mitochondria. These proteins interact with pro-apoptotic proteins and regulate the intrinsic apoptotic pathway by controlling the mitochondrial membrane permeability and release of the pro-apoptotic factor; Cytochrome c which initiates activation of the Caspase cascade and apoptosis.
Eurofins DiscoverX now offers a novel, direct, cell-based assays to study these apoptotic players. No more assay development required; you can obtain pre-validated biosensor cell lines that allows you to screen for protein interaction inhibitors (small molecules or siRNA) in a HTS-friendly and simple assay format!
How it Works
Features and Benefits
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Simple, HTS-friendly protocol
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Whole cell, chemiluminescent assay
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Avoid antibodies and lengthy FLOW based assay development
Applications
Assay List
Cell Lines
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Target
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Description
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Cat. No.
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Bcl2-Bax
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InCELL Hunter™ U2OS Bcl2-Bax Protein Binding Cell Line
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96-0010C3
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Bcl2-Bim
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InCELL Hunter™ U2OS Bcl2-Bim Protein Binding Cell Line
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96-0008C3
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Bcl2L1-Bax
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InCELL Hunter™ U2OS Bcl2L1-Bax Protein Binding Cell Line
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96-0009C3
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Bcl2L1-Bim
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InCELL Hunter™ U2OS Bcl2L1-Bim Protein Binding Cell Line
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96-0037C3
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Assay Ready Kits
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Target
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Description
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Cat. No.
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Bcl2L1-Bax
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InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay
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96-0009E3CP16L
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Bcl2L1-Bax
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InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay
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96-0009E3CP16M
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