Inhibition of Serotonin (5-Hydroxytryptamine) HTR2B by Reference Compounds
Cells were stimulated with reference compounds as shown in the figure. Following stimulation, calcium mobilization was detected using the DiscoverX’s HitHunter Calcium No WashPLUS
detection kit (Cat. No. 90-0091) and IC50
Inhibition of Cyclooxygenase COX2 by Reference Compounds
COX2 enzyme activity was determined by measuring the conversion of arachidonic acid to PPG2 based on fluorometirc readout. COX enzyme was incubated with the compounds at room temperature for 30 minutes before addition of the arachidonic acid (1.7 uM) and Ampliflu Red (2.5 uM). Plate was read on a fluorimeter with the emission detection at 590 nm with excitation wavelength 544 nm.