Assay Performance
Ideal for Screening and Profiling
The simplicity and versatility of the PathHunter® NHR
TRANS and NHR
PRO assays make these assays ideal for screening and profiling to look for agonists, antagonists, inverse agonists.
Profiling of Functional, Cell-Based Nuclear Hormone Receptors
ERRα ERRα GR
Agonist Mode Inverse Agonist Mode Antagonist Mode
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Agonist (Left) and Inverse agonist (Middle) profiling of Estrogen related receptor alpha, ERRα and antagonist profile of glucocorticoid receptor, GR (Right).
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nhrE/IC50ELECT Assay Panel
Listed below are the assays currently available for screening and profiling.
| Target Gene ▲ | Common Name | Protein Interaction |
| AR | Androgen receptor (AR) | X |
| ERα | Estrogen receptor 1 (ESR1) | X |
| FXR | Nuclear receptor subfamily 1, group H, member 4 (NR1H4) | X |
| GR | Nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) (NR3C1) | X |
| LXRα | Nuclear receptor subfamily 1, group H, member 3 (Liver X receptor α) (NR1H3) | X |
| LXRβ | Nuclear receptor subfamily 1, group H, member 2 (Liver X receptor β) (NR1H2) | X |
| LXRβ-NCoR1 | Nuclear receptor subfamily 1, group H, member 2 (Liver X receptor β) (NR1H2) | X |
| MR | Nuclear receptor subfamily 3, group C, member 2 (Mineralcorticoid receptor) (NR3C2) | X |
| PPARα | Peroxisome Proliferator Activated Receptor alpha (PPARA) | X |
| PPARγ | Peroxisome Proliferator Activated Receptor gamma (PPARG) | X |
| PPARδ | Peroxisome Proliferator Activated Receptor delta (PPARD) | X |
| PRα | Progesterone receptor α (PGR) | X |
| PRβ | Progesterone receptor β (PGR) | X |
| RARα | Retinoic acid receptor, alpha (RARA) | X |
| RXRα | Retinoid X receptor, alpha (RXRA) | X |
| RXRγ | Retinoid X receptor, gamma (RXRG) | X |
| THRα | Thyroid hormone receptor, alpha (THRA) | X |
| THRβ | Thyroid hormone receptor, beta (THRB) | X |
Data Analysis & Interpretation
What does the term EC50 mean?
The term half maximal effective concentration (EC50) refers to the concentration of a drug or biologic which induces a response halfway between the baseline and the maximum. Therefore, the EC50 of a compound or biologic represents the concentration where 50% of it’s maximal effect can be observed. It is commonly used as a measure of the drug’s potency.
What does an IC50 measure?
The term half maximal inhibitory concentration (EC50) is a measure of the effectiveness of a compound to inhibit a given biological response. Therefore, the IC50 of a compound or biologic represents the half maximal (50%) inhibitory concentration and is commonly used as a measure of antagonist drug’s potency.
How are the EC50/IC50s calculated?
For each compound/GPCR target interaction, dose response curves are generated using 10 concentrations of agonist or antagonist (10-point curve). The results are reported as EC50/IC50 which derived using the Hill equation:
