Multi-mode Screening and Profiling
With nhrDUO, you can screen or profile compounds using the nuclear translocation and protein interaction readouts in parallel. Quantitatively examining these pathways in parallel can uncover potency and efficacy differences between compounds and aid in defining compound function to better predict compound activity in vivo.
GR NHRTRANS Assay
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GR NHRPRO Assay
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The PathHunter GR Nuclear translocation (NHRTRANS) assay shows no inhibition after addition of Mifepristone (Left) while the GR protein interaction (NHRPRO) assay shows almost complete inhibition of the Dexamethasone response (Right), indicating that Mifepristone acts on the target of interest by blocking co-activator recruitment.
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nhrDUO Assay Panel
Listed below are the assays currently available for screening and profiling.
Data Analysis & Interpretation
Definitions
Percent Activity/Inhibition
The results for single concentration (primary screen) for tested compound(s) are reported in your study report and spreadsheets as '% Activity' or '% Inhibition' and are calculated in the following manner:
% Activity
(Agonist Mode) =
100% x
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Mean RLU (test sample)- Mean RLU(vehicle control)
Mean MAX RLU (control ligand)- Mean RLU (vehicle control)
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test sample = client supplied compound
vehicle control = DMSO (0% activity)
control ligand = control compound (100% activity)
% Inhibition
(Antagonist mode) =
100% x
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1- |
Mean RLU (test sample)- Mean RLU(vehicle control)
Mean MAX RLU (control ligand)- Mean RLU (vehicle control)
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] |
test sample = client supplied compound
vehicle control = DMSO (0% activity)
EC80 control = control compound (80% activity)
Dose Response Curve (EC50/IC50)
The results for 10-point dose response curve compound/target interactions are reported in the study report and spreadsheets as EC50/IC50, which are values derived using the Hill equation:
