Detection Reagents - Ultrasensitive, Robust & Reliable

DiscoverX’s detection reagents are based on our proprietary Enzyme Fragment Complementation (EFC) technology, which provides high sensitivity detection for a variety of applications such as detecting cell-based protein-protein interactions, compound-protein binding, degradation, and translocation.  These reagents are used in conjunction with cAMP Hunter™, InCELL Hunter® or PathHunter® cell lines, InCELL assays, and ProLabel®/ProLink™ expression or cloning vectors inside whole cells. They allow for miniaturization and detection of enzyme fragment complementation or just the ProLink or ProLabel epitopes.

Product Features & Benefits

  • Simple one-step addition
  • Robust & Reproducible detection
  • Compatible with 96-well, 384-well, 1536 and 3456 formats
  • Read on any standard benchtop luminometer

Detection Reagent Calculator

Detection Reagents

Cat. No. Description Size
90-0075-LM2 HitHunter® cAMP Assay for Biologics 200/800 dp (96-well/384-well)
90-0075-LM25 HitHunter® cAMP Assay for Biologics 2,500/10,000 dp (96-well/384-well)
90-0075-LM10 HitHunter® cAMP Assay for Biologics 1,000/4,000 dp (96-well/384-well)
90-0075-SM2 HitHunter® cAMP Assay for Small Molecules 200/800 dp (96-well/384-well)
90-0075-SM10 HitHunter® cAMP Assay for Small Molecules 1,000/4,000 dp (96-well/384-well)
90-0075-SM25 HitHunter® cAMP Assay for Small Molecules 2,500/10,000 dp (96-well/384-well)
96-0079S InCELL Detection Kit 54 mL (4 plates), 400/1,600 dp (96-well/384-well)
96-0079 InCELL Detection Kit 216 mL (16 plates), 1,600/6,400 dp (96-well/384-well)
96-0079L InCELL Detection Kit 2,000 mL (100 plates), 40,000 dp (384-well)
97-0001S KILR® Detection Kit 200 dp (96-well)
97-0001M KILR® Detection Kit 1,000 dp (96-well)
97-0001L KILR® Detection Kit 2,500 dp (96-well)
93-0933 PathHunter® Bioassay Detection Kit 50 mL (1,000 dp, 96-well)
93-0933L PathHunter® Bioassay Detection Kit 500 mL (10,000 dp, 96-well)
93-0933E PathHunter® Bioassay Detection Kit 10 mL (200 dp, 96-well)
93-1043E PathHunter® Bioassay ED Detection Kit 10 mL (200 dp, 96-well)
93-1043 PathHunter® Bioassay ED Detection Kit 50 mL (1,000 dp, 96-well)
93-0001M PathHunter® Detection Kit 15 mL (2 plates), 200/800 dp (96-well/384-well)
93-0001S PathHunter® Detection Kit 7.5 mL (1 plate), 100/400 dp (96-well/384-well)
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well)
93-0001L PathHunter® Detection Kit 550 mL (100 plates), 40,000 dp (384-well)
93-0183 PathHunter® EA Detection Kit 800 dp (384-well)
93-0247 PathHunter® Flash Detection Kit 125 mL (10 plates), 1,000/4,000 dp (96-well/384-well)
93-0247L PathHunter® Flash Detection Kit 1,000 mL (100 plates), 40,000 dp, (384-well)
93-0247S PathHunter® Flash Detection Kit 12.5 mL (1 plate), 100/400 dp (96-well/384-well)
93-0247M PathHunter® Flash Detection Kit 25 mL (2 plates), 200/800 dp (96-well/384-well)
93-0247XL PathHunter® Flash Detection Kit 4,000 mL (400 plates), 160,000 dp, (384-well)
93-0812S PathHunter® PL/PK Detection kit 400 dp (384-well)
93-0812 PathHunter® PL/PK Detection kit 4,000 dp (384-well)
93-0812L PathHunter® PL/PK Detection kit 40,000 dp (384-well)
93-0180 PathHunter® ProLabel® Detection Kit 800 dp (384-well)
93-0180L PathHunter® ProLabel® Detection Kit 10,000 dp (384-well)
92-0006 ProLink™ Detection Kit 800 dp (384-well)

Refer to selection guide tables below to select a Detection kit that's right for you.

Detection kit compatibility with DiscoveRx cell lines

Detection Kit Name Cat No. Compatible Cell Line Product Categories  Cell Line Target
InCELL Hunter™ Detection Kit 96-0002 InCELL Hunter™ Epigenetic Assays View Targets
InCELL Hunter™ Detection Kit II 96-0039 InCELL Hunter™ Kinase Assays View Targets
PathHunter® Detection Kit 93-0001 PathHunter® β-Arrestin Human View Targets
PathHunter® β-Arrestin Ortholog View Targets
PathHunter® Cytosolic Tyrosine Kinase View Targets
PathHunter® GPCR Internalization - Active View Targets
PathHunter® GPCR Internalization - Total View Targets
PathHunter® GPCR Internalization - Total MEM View Targets
PathHunter® β-Arrestin Orphan View Targets
PathHunter® Pathway View Targets
PathHunter® Nuclear Hormone (PI) View Targets
PathHunter® Nuclear Hormone (NT) View Targets
PathHunter® Cell-Based Kinase View Targets
PathHunter® Cell-Based Kinase Internalization View Targets
HitHunter® cAMP Assay 90-0075 cAMP Hunter™ Human View Targets
cAMP Hunter™ Ortholog View Targets
Calcium No WashPLUS (CaNW) Assay Kit 90-0091 Native Calcium Gq Coupled Cell Lines View Targets
PathHunter® PK/PL Detection Kit 93-0812 PathHunter® Pathway Bcl2-Bim
PathHunter® Flash Detection Kit 93-0247 PathHunter® Pathway Cdc25a
HitHunter® cGMP Assay 90-0039 PathHunter® Pathway NPR1

Detection kits for functional confirmation of EFC component expression in cell lines

Detection Kit Name Cat No. Intended Use
EFC BlocDetect™ Kit 92-0004 Detect false positive results by identifying compounds that inhibit β-galactosidase complementation or β-galactosidase activity
ProLink™ Detection Kit 92-0006 Detect ProLink™-tagged protein in parental cells expressing ProLink™ Vectors
PathHunter® ProLabel Detection Kit 93-0180 Detect ProLabel™-tagged protein in parental cells expressing ProLabel™ Vectors
PathHunter® EA Detection Kit 93-0183 Detect large protein fragment of β-galactosidase (Enzyme Acceptor, EA) in parental cells expressing EA (EA-Nuc, EA-Cyto, or EA-β-Arrestin)