PathHunter® β-Arrestin Assays
Universal Assays for Ligand Bias, Receptor Deorphanization, and Antagonist Discovery
Universal G-protein independent assays to easily quantify GPCR activation based on the recruitment of β-arrestin. Ideal for antagonist mode screening and studying virtually any GPCR including orphan receptors. Multiplex with calcium or cAMP readouts for cost-effective screening and ligand bias analysis.
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ChemiScreen™ Membrane Preps
High Quality Membrane Preps for Radioligand Binding and Functional GTPγS Assays
Utilize high-quality membrane preparations to identify ligands that bind your GPCR. Membrane preps are available for saturation and competitive radioligand binding and GTPγS functional studies. Obtain consistent, reproducible results and high total binding values with large signal-to-background ratio.
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cAMP Cell Lines, Frozen Cells, and Kits
Complete assay solutions for monitoring cAMP
Monitor the functional status of GPCRs directly by quantifying cellular cAMP accumulation levels in a dose-dependent manner. Characterize ligand pharmacology with precision. Reproducible performance with large assay windows and broad sensitivity ranges. Simple assays with options for chemiluminescent or fluorescent readout.
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Calcium Cell Lines, Frozen Cells, and Kits
Directly measure calcium flux
- Measure intracellular calcium mobilization in cells based on the activation status of GPCRs or ion channels. Cell lines and frozen cells qualified for use with FLIPR® or dye-based assays with fluorescent read-out. Robust and flexible assays with broad sensitivity ranges and large assay windows with protocols for adherent or suspension cells.
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PathHunter GPCR Internalization Assays
Quantitative secondary and orthogonal screening assays for identifying safer drugs
Measure GPCR internalization in live cells with chemiluminescent functional assays as a primary and orthogonal screening tool to help in identifying safer drugs. Quantify internalized GPCRs localized to intracellular endosomes. Monitor your activated GPCR in live cells without expensive microscopy, bulky labels, or antibodies.
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PathHunter Pharmacotrafficking Assays
Simple assays to identify and optimize pharmacochaperone and study GPCR trafficking to the membrane or endosome.
Identify and characterize small molecule compounds that enter the cell, function as pharmacological chaperones by promoting proper folding, and assist in trafficking to their intended location. Interrogate your compounds’ function in disease processes associated with protein trafficking and internalization due to protein misfolding. Quantitative chemiluminescent results without imaging.
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PathHunter β-Arrestin Parental Cell Lines &Retroparticles
Create Your Own Target-Specific, Stable β-Arrestin Cell-Based Assays
Make your own stable β-arrestin cell lines and EFC cell-based assays for any target in any dividing cell type. Use the assays to analyze the activation and desensitization of GPCRs and regulation of other signaling molecules such as protein kinases that involve β-arrestin recruitment.
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Custom Assay Development Services
Custom Assays and Stable Cell Lines We Can Develop For You
When existing off-the-shelf solutions are not enough, trust Eurofins Discovery to develop a solution that will produce the quality results you expect. These services include custom assays with unique cell backgrounds, orthologs, and mutants as well as creation of custom cellular or biochemical assays for drug discovery, process development, and basic research.
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