Anti-drug Antibody Bioassays -

Applications & Products

Cell-Based Assays to Detect Neutralizing Anti-Drug Antibodies (ADAs)

Sensitive detection of NAbs in up to 100% human serum

PathHunter® assays are widely used to detect neutralizing anti-drug antibodies (ADAs) or NAbs in patient serum samples, with high sensitivity and reproducibility. These assays use a simple, mix-and-read protocol that rapidly detects neutralizing antibodies in the presence of high concentrations of human serum. These optimized assays provide superior performance for a quick, successful regulatory submission data package, increasing quality and success of any program.

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View List of Targets

Tolerance of Human Serum by PathHunter Assays

HRH1: Human Serum Effect

CMKLR1: Human Serum Effect

PathHunter assays detect functional biologics and neutralizing antibodies in high concentrations of human serum. The tolerance of the assay to human serum depends on the target and its inherent biology. For targets that do not have a natural agonist in normal human serum, the assay is very tolerant of high concentrations of serum because the assays provides a highly target specific response, e.g. HRH1 assay, (left panel). In other cases, increasing serum concentration might decrease the assay window due to an unexplained increase in background activity, e.g. CMKLR1 assay, (right panel).

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Functional Cell-Based Assays To Identify Neutralizing Antibodies for Immunogenicity Testing

Features of PathHunter Cell-Based Assays for Immunogenicity Testing

Tolerance to high concentrations of normal human serum

High sensitivity for low antibody concentrations

Rapid results; simple protocol

High reproducibility

Frozen, thaw-and-use format available(Bioassay Kits)

Target-Specific and Highly Sensitive Response

IGFR Assay Specificity

IGF1R Assay with Anti-IGF1

Highly Target Specific And Sensitive Assay. PathHunter assays provide a highly target specific and sensitive response to ligand. IGF1R assay (left panel) with a variety of agonists and only observed a response with the IGF1 protein. Above right, we tested the IGF1R assay with anti-IGF1 antibody and the ligand. The assay sensitivity to the antibody response was 440ng/ml.

IGFR Assay Specificity		IGF1R Assay with Anti-IGF1

Highly Target Specific And Sensitive Assay. PathHunter assays provide a highly target specific and sensitive response to ligand. IGF1R assay (left panel) with a variety of agonists and only observed a response with the IGF1 protein. Above right, we tested the IGF1R assay with anti-IGF1 antibody and the ligand. The assay sensitivity to the antibody response was 440ng/ml.