PathHunter assays detect functional biologics and neutralizing antibodies in high concentrations of human serum. The tolerance of the assay to human serum depends on the target and its inherent biology. For targets that do not have a natural agonist in normal human serum, the assay is very tolerant of high concentrations of serum because the assays provides a highly target specific response, e.g. HRH1 assay, (left panel). In other cases, increasing serum concentration might decrease the assay window due to an unexplained increase in background activity, e.g. CMKLR1 assay, (right panel).
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