HitHunter® cAMP Assay Product Change Notice
| Effective January 12, 2015, the historic kit, HitHunter cAMP XS+ will be changing to two new application specific kits. |
Historical Kit
HitHunter cAMP XS+ (Prod. No. 90-0075S, 90-0075, 90-0075L, 90-0075XL)
New Application Specific Kits
HitHunter cAMP Assay for Biologics (Prod. No. 90-0075LM series)
HitHunter cAMP Assay for Small Molecules (Prod. No. 90-0075SM series)
|
| Changes Made |
Identical to Previous Kit |
| Optimized protocol by application |
Expected results |
| Changed kit sizes and additional reagent volume |
Reagent formulations and components |
| New product numbers and names |
Technology principle |
| Redesigned user manual |
General procedure |
Summary of Changes
Product Names, Numbers and Kit Sizes
Bulk quantities are available upon request
| Application Specific Kits |
| HitHunter cAMP Assay for Biologics |
HitHunter cAMP Assay for Small Molecules |
| Product No. |
Size Configuration |
Product No. |
Size Configuration |
| 90-0075LM2 |
200/800 dp (96-well/384-well) |
90-0075SM2 |
200/800 dp (96-well/384-well) |
| 90-0075LM10 |
1,000/4,000 dp (96-well/384-well) |
90-0075SM10 |
1,000/4,000 dp (96-well/384-well) |
| 90-0075LM25 |
2,500/10,000 dp (96-well/384-well) |
90-0075SM25 |
2,500/10,000 dp (96-well/384-well) |
User Manual Changes
- Updated general format and layout of the manual to a new design that incorporates additional images and clearer instructions in an easy-to-follow step-by-step process
- FAQs and user important notes as call-outs throughout user manual have been incorporated
- Updated the typical results and protocol flowchart (quick-start procedure)
- Included an appendix section with protocol modifications for antagonists, crude biologics, positive allosteric modulators (PAMs), negative allosteric modulators (NAMs), and other protocol variation
Protocol Comparison
The figures below show a comparison of results using the HitHunter cAMP XS+ kit and the New HitHunter cAMP Assays. Results in both figures indicate reproducible EC50 and signal to background values when run side by side.