Most Robust cAMP Assays for Small Molecules and Biologics

Sensitive Assays with Unmatched Performance

The HitHunter® cAMP Assays provide a robust, easy-to-use cell-based detection system for monitoring GPCR activation based on cAMP production in cells. Monitor functional status of Gαi- and Gαs-coupled receptors directly by quantifying cellular cAMP levels in a dose-dependent manner, enabling you to conduct functional screens and optimize biologics or small molecule compounds. See How it Works
View Validation Data

Features and Benefits

  • Flexible – Use with small molecules or biologics
  • Validated – In 400+ peer reviewed publications
  • Qualified – Unmatched performance for a variety of applications
  • Robust – Exquisite sensitivity with large assay windows

Available Products

View the available HitHunter cAMP Assays (detection reagents) specific to small molecules and biologics. Choose a complimentary cAMP Hunter™ cell lines to use with the HitHunter cAMP Assays. Alternatively, select from 100s of assay ready cAMP Hunter eXpress kits, which include a specific cell line and detection reagents.
 
Detection Reagents Cell Lines Assay Ready Kits


Optimal for a Variety of Applications

Rank Molecule Potency


 

Measure Complex Assay Formats

 


Obtain Excellent Reproducibility



Characterize Biologics


HitHunter cAMP assays can be used for multiple applications. (top-left) Profiling experiment of ranked adrenergic receptor β2 agonists showing assay can obtain the expected pharmacology. (top-right) Comparison study of two antagonists for the Gαi-coupled metabotropic glutamate receptor 2 indicates the ability of the assay to measure complex assay formats. (bottom-left) Evaluation of lot-to-lot consistency using a GLP agonist and the GLPR1 cell line indicates excellent reproducibility. (bottom-right) Characterization of the biologic, SDF1α, using the CXCR4 Gαi-coupled receptor cell line.
 
Additional Applications
Perform functional screens using small molecules or biologics (e.g. antibodies, peptides) Detect neutralizing antibodies (immunogenicity studies using serum and plasma)
Identify PAMs, NAMs, partial agonists, inverse agonists, and silent antagonists Run quality control assays (potency assays for lot release and stability testing)
Characterize phosphodiesterase inhibitors Quantify low levels of cAMP (endogenous receptor studies)