Biological Information

Background Information:

The PathHunter® U2OS TrkA Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkA dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged TrkA and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TRKA

Protein Aliases:

TRK1-transforming tyrosine kinase protein|Tropomyosin-related kinase A|Tyrosine kinase receptor|Tyrosine kinase receptor A|Trk-A|gp140trk|p140-TrkA|high affinity nerve growth factor receptor

Accession Number:

NM_002529

UniProt Number:

P04629

Gene Name:

NTRK1

Gene ID:

4914

Gene Aliases:

TRK|TRKA|MTC

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Functional Activity

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Oncology/Immuno-Oncology

Additional Information

Brand:

PathHunter®