PathHunter® U2OS NF-KB Reporter Cell Line

The PathHunter® U2OS NF-kB Pathway Reporter Cell Line measures NF-kB pathway activation through detection of induced expression levels of a tagged reporter protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1157C3 Size: 2 vials
Inquire
Biological Information
Background Information:
The PathHunter® U2OS NF-kB Pathway Reporter Cell Line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting the NF-kB pathway. This cell line enables assessment of NF-kB pathway stimulation based on changes in the expression of a NF-Kb response element-driven reporter protein. This product uses EFC technology. The included cell line expresses PL-tagged reporter protein under the control of a NF-KB pathway-inducible transcriptional response element. Pathway activation induces expression of the PL-tagged protein, allowing detection of EFC signal upon addition of EA-containing detection reagent.  This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Signaling Pathway
Target Name:
NF-kB
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Signaling Reporters
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Signaling
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-406 PH Signaling Pathway Reporter Assay User Manual REV0

View Document

Datasheets

93-1157C3

View Document
PathHunter® PL/PK Detection kit
93-0812
Inquire
AssayComplete™ Cell Culture Kit-103
92-3103G
Kit

$397.00

AssayComplete™ Cell Plating 3 Reagent
93-0563R3A
100 mL

$339.00

AssayComplete™ Thawing Reagent T3
92-4103TR
100 mL

$249.00

AssayComplete™ Cell Detachment Reagent
92-0009
50 mL

$109.00

AssayComplete™ Freezing Reagent F3
92-5103FR
2 x 50 mL

$339.00