PathHunter® U2OS EphA4 Functional Assay

Background Information:

The PathHunter® U2OS EphA4 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphA4 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged EphA4 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

EphA4

Protein Aliases:

EPH-like kinase 8|EK8|hEK8|Tyrosine-protein kinase TYRO1|Tyrosine-protein kinase receptor SEK

Accession Number:

NM_004438.3

UniProt Number:

P54764

Gene Name:

EPHA4

Gene ID:

2043

Gene Aliases:

Hek8

Target Species:

Human

Cell Background:

U2OS