PathHunter® Human EphA4 Functional Kinase Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human EphA4 Functional Kinase Cell-Based Assay Kit (U2OS) utilizes the U2OS EphA4 Functional Assay Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting receptor tyrosine kinases (RTKs). This kit assesses RTK activation via the detection of EphA4 activation-induced SH2 domain protein recruitment. The included cell line overexpresses PK-tagged Human EphA4 and an EA-tagged SH2 domain protein. Upon ligand (e.g. ephrin A3)-induced receptor activation, SH2-EA recruitment leads to the production of an EFC signal proportional to the degree of receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

EphA4

Protein Aliases:

EPH-like kinase 8|EK8|hEK8|Tyrosine-protein kinase TYRO1|Tyrosine-protein kinase receptor SEK

Accession Number:

NM_004438.3

UniProt Number:

P54764

Gene Name:

EPHA4

Gene ID:

2043

Gene Aliases:

Hek8

Target Species:

Human

Cell Background:

U2OS