PathHunter® P2RY12 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® P2RY12 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. ATP) based internalization of P2RY12 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged P2RY12 and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the P2RY12 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

P2Y

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

P2Y12

Protein Aliases:

ADP-glucose receptor|ADPG-R|P2T|AC|P2Y|AC|P2Y|cyc|P2Y12 platelet ADP receptor|P2Y|ADP|SP1999

Accession Number:

AY136754

UniProt Number:

Q9H244

Gene Name:

P2RY12

Gene ID:

64805

Gene Aliases:

P2Y12|SP1999|HORK3

Target Species:

Human

Cell Background:

U2OS