PathHunter® Human P2RY12 Stable Cell Line Total GPCR Internalization Assay (U2OS)

Background Information:

The PathHunter Human P2RY12 Stable Cell Line Total GPCR Internalization Assay (U2OS) utilizes the P2RY12 Total GPCR Internalization cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g., ATP) based internalization of P2RY12 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged P2RY12 and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, the internalization of the P2RY12 receptor to the early endosome results in the production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

P2Y

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

P2Y12

Protein Aliases:

ADP-glucose receptor|ADPG-R|P2T|AC|P2Y|AC|P2Y|cyc|P2Y12 platelet ADP receptor|P2Y|ADP|SP1999

Accession Number:

AY136754

UniProt Number:

Q9H244

Gene Name:

P2RY12

Gene ID:

64805

Gene Aliases:

P2Y12|SP1999|HORK3

Target Species:

Human

Cell Background:

U2OS