PathHunter® Jurkat PD-1 (SHP1) Signaling Assay

The PathHunter® Jurkat PD-1 (SHP1) Signaling Assay measures PD-1 signal by recruitment of SH2 domain protein to the activated receptor. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1104C19 Size: 2 vials

Biological Information
Background Information:	The PathHunter® PD-1 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. PD-L1, PD-L2) based activation of PD-1 receptors via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PD-1 target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Checkpoint Receptor
Family:	IgSF (immune inhibitory Receptor)
Accession Number:	NM_005018.2
Target Name:	PD-1
Target Aliases:	PDCD1Programmed cell death protein 1PD1PD-1CD279SLEB2hPD-1hPD-lhSLE1
Target Species:	Human
Cell Background:	Jurkat

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Short term (24 h): Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Signaling
Detection Method:	Chemiluminescence

Clinical Relevance
Therapeutic Area:	Oncology/Immuno-Oncology

Additional Information
Brand:	PathHunter®