The PathHunter® PD-1 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting inhibitory checkpoints. This cell line enables assessment of ligand (e.g. PD-L1, PD-L2) based activation of PD-1 receptor via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PD-1 target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of chemiluminescent signal.
This cell line can be used to evaluate both agonist and antagonist antibodies targeting PD-1 receptor. Running the assay in agonist mode may require the use of Fcg Receptors expressing cells to enable clustering.
This stable engineered cell line is provided as two vials of cryopreserved cells.