| Biological Information | |
|---|---|
| Background Information: | The PathHunter® EDNRB Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. endothelin-3) based internalization of EDNRB via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged EDNRB and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDNRB receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Endothelin Receptors |
| Sub Family: | Class A |
| Coupling: | Gs & Gi/Go |
| Accession Number: | NM_000115.2 |
| Target Name: | EDNRB |
| Target Aliases: | Endothelin receptor type B, ABCDS, ET-B, ET-BR, ETB, ETB1, ETBR, ETRB, HSCR, HSCR2, WS4A |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Internalization |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress EDNRB Total GPCR Internalization Assay
The PathHunter® eXpress EDNRB Total GPCR Internalization Assay measures EDNRB (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-262 PathHunter eXpress Total GPCR Internalization Assays REV7 (1) User Manual
View DocumentDatasheets
93-0874E3x Datasheet
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