PathHunter® eXpress EDNRB Total GPCR Internalization Assay

Background Information:

The PathHunter® EDNRB Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. endothelin-3) based internalization of EDNRB via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged EDNRB and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDNRB receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Endothelin

Sub Family:

Class A

Coupling:

Gs & Gi/Go

Protein Name:

ETB

Protein Aliases:

ET-BR|Endothelin receptor non-selective type

Accession Number:

NM_000115.2

UniProt Number:

P24530

Gene Name:

EDNRB

Gene ID:

1910

Gene Aliases:

ETB

Target Species:

Human

Cell Background:

U2OS